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. 2016 Apr 25;5:137. Originally published 2016 Feb 4. [Version 2] doi: 10.12688/f1000research.7800.2

Figure 1. Tau and TDP-43 levels were not increased when coexpressed with V5-tagged hUSP14wt versus V5-tagged hUSP14(CA).

Figure 1.

1, 1.5 or 2ug of V5-hUSP14wt (wt = wild type) or V5-hUSP14(CA) (CA = C114A, catalytically inactive) were cotransfected with 2ug Tau or Flag-TDP-43 plasmid in HEK293 cells. Cells were lysed after 48 hours and analyzed by western blot using a standard protocol. Actin served as loading control. Despite robust expression of USP14 or its catalytically inactive mutant as detected by the V5-tag ( A, C), no differences were observed in Tau ( A, B) or Flag-TDP-43 ( C, D) protein levels. Note that we did not observe differences in the expression levels of USP14 versus USP14(CA). Control = empty vector control.