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. 2016 Apr 25;5:137. Originally published 2016 Feb 4. [Version 2] doi: 10.12688/f1000research.7800.2

Figure 2. No decreases observed in tau or TDP-43 levels after cotransfection with untagged hUSP14(CA) versus untagged hUSP14wt.

Figure 2.

31 to 500ng of hUSP14wt or hUSP14(CA) plasmids were cotransfected with 2ug tau or TDP-43 plasmid in HEK293 cells. Cells were lysed after 48 hours and analyzed by western blot using a standard protocol. Actin served as loading control. Despite robust expression of USP14 or the catalytically inactive mutant as detected by anti-USP14 antibody ( A, C), no decreases were observed in tau ( A, B) or TDP-43 ( C, D) protein levels in the cells transfected with hUSP14CA. Note that we did not observe differences in the expression levels of USP14 versus USP14(CA). Mock = empty vector control, GOI = gene of interest and refers to either tau or TDP-43 in the absence of USP14 cotransfection.