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. 2016 Jan 15;30(2):234–247. doi: 10.1210/me.2015-1281

Figure 3.

Figure 3.

Effects of recombinant proteins on cholesterol movement to mitochondria. A and B, Effect of StAR on cholesterol movement to mitochondria. C, Confocal image of StAR-mediated cholesterol movement to mitochondria (100 times). Mitochondria (red) were labeled with MitoTracker Red FM (Invitrogen), and cholesterol (green) was labeled with bodipy-CE. Scale bar, 2 μm. D and E, Effects of GTP and GTPγs on SNARE function. F, Comparison of recombinant SNARE proteins and cytosol on cholesterol movement to mitochondria. In panels A, B, D, and E, mitochondria were pretreated with trilostane (10 μM) for 2.5 minutes at room temperature before being added to the reconstitution assay. In panel D, mitochondria were pretreated with trilostane (5 μM) and abiraterone (10 μM) for 2.5 minutes at room temperature before being added to the reconstitution assay. Pregnenolone was measured by an ELISA. *, P < .05; **, P < .01; ***, P < .001. All, cocktail containing all recombinant SNARE proteins; cyto, cytosol; LE, lipid emulsion; M, mitochondria. Results are representative of three to five independent experiments, each with an n = 3.