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. 2016 Jan 15;30(2):234–247. doi: 10.1210/me.2015-1281

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Figure 4. — Effects of individual recombinant SNARE proteins on cholesterol movement to mitochondria. A, Effect of α-SNAP on cholesterol movement to mitochondria. B, Effect of SNAP25 on cholesterol movement to mitochondria. C, Effect of Stx17 on cholesterol movement to mitochondria. D, Effect of NSF on cholesterol movement to mitochondria. E, Effect of SNAP23 on cholesterol movement to mitochondria. Mitochondria were pretreated with trilostane (5 μM) and abiraterone (10 μM) for 2.5 minutes at room temperature before being added to the reconstitution assay. Pregnenolone was measured by ELISA. *, P < .05; **, P < .01; ***, P < .001. All, cocktail containing all recombinant SNARE proteins; LE, lipid emulsion; M, mitochondria. Results are representative of three independent experiments, each with an n = 3–4.