Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 21;30(2):189–200. doi: 10.1210/me.2015-1186

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article has been published under the terms of the Creative Commons Attribution License (CC-BY; https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s).

PMC Copyright notice

Figure 2. — Estrogen responsiveness of ERE-WT and ERE-Mut BAC cell lines. E2 stimulation (10nM) of 5 individual serum-starved (24 h) ERE-WT and ERE-Mut clones; after treatment for 24 hours with either 10nM E2 or vehicle control (0.1% dimethylsulfoxide [DMSO]), cells were lysed and assayed for Luc activity; **, P < .001 (A). Effect of E2 cotreatment with the E2 antagonist 4-OHT (1000nM) in ERE-WT1 and ERE-Mut3 cell lines (B). Live cell luminometry of serum-starved (24 h) ERE-WT1 (C) and ERE-Mut3 (D) cell lines using increasing concentrations of E2 with Luc activity measured every 15 minutes. The dotted line indicates the baseline determined from untreated control samples. Results are shown as fold induction ± SD from the untreated control sample with values representing the mean of at least 3 independent experiments, each with 3 replicates.