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. 2016 Mar 3;6:22176. doi: 10.1038/srep22176

Figure 2. MICALMO-CH is an intact molecule in solution and in the crystal.

Figure 2

(a) Molecular weight characterized by size exclusion chromatography MICALMO-CH eluted as a monomer of 61.4 kDa (red line) in a size exclusion chromatography using a Superose-12 10/300GL (GE HealthCare) column. Standards are shown in blue, with the molecular weights (Mw) in kDa indicated. (b) Logarithmic plot of the Mw for the standards the ratio between elution volume (Ve) and column exclusion volume (Vo). The pink diamond mark indicates the MICALMO-CH elution volume observed. (Inset) SDS-PAGE analysis of MICALMO-CH crystals. Lane 1: crystals dissolved in SDS running buffer after washing with crystallization mother liquor; lane 2: 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100, 110 kDa molecular weight ladder and lane 3: MICALMO-CH after purification.