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. 2016 Mar 3;6:22176. doi: 10.1038/srep22176

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Copyright © 2016, Macmillan Publishers Limited

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(a) Rapid equilibrium diagrams for the system enzyme (MICAL_X)/substrate (NADPH)/activator (F-actin). (Left) for MICAL_MO and (right) for MICAL_MO-CH. The equilibrium constants and rates in each branch are shown with their SE calculated from the non-linear fit of Equation. [1] to the initial rate of NADPH oxidation by MICAL_MO and MICAL_MO-CH in presence of F-actin. (b) Steady state velocities and their fitting with Equation (1) (color traces) and Equation (2) (black traces). The symbol error-bars represent the standard error of the mean SEMs. (c) Apparent and of both constructs obtained by the fitting the initial velocities for each value of [F-actin] with equation (2). (d) Parameters of the global fitting of MICAL_MO and MICAL_MO-CH using the non-essential activator (F-actin) Equation (1), the parameter α was constrained to 1 in the MICAL_MO dataset. Error bars represent standard errors (SEs). All fitting and statistical tests were performed using the program Prism 6 (GraphPad Inc.).

Inline graphic — (a) Rapid equilibrium diagrams for the system enzyme (MICAL_X)/substrate (NADPH)/activator (F-actin). (Left) for MICAL_MO and (right) for MICAL_MO-CH. The equilibrium constants and rates in each branch are shown with their SE calculated from the non-linear fit of Equation. [1] to the initial rate of NADPH oxidation by MICAL_MO and MICAL_MO-CH in presence of F-actin. (b) Steady state velocities and their fitting with Equation (1) (color traces) and Equation (2) (black traces). The symbol error-bars represent the standard error of the mean SEMs. (c) Apparent and of both constructs obtained by the fitting the initial velocities for each value of [F-actin] with equation (2). (d) Parameters of the global fitting of MICAL_MO and MICAL_MO-CH using the non-essential activator (F-actin) Equation (1), the parameter α was constrained to 1 in the MICAL_MO dataset. Error bars represent standard errors (SEs). All fitting and statistical tests were performed using the program Prism 6 (GraphPad Inc.).