Figure 2. Evaluation of clinical signs and viral replication following inoculation of H1N1 viruses in ferrets.
Groups of 3 ferrets were inoculated i.n. with 106 TCID50/0.2ml of H1N1 CA/7/09 (●), CA/4/09 (×), NJ/76 (□), sw/IA/31(○) viruses. Mock-infected ferrets (◇) received L-15. Percentage change in body weight (A) and average body temperature (B) following i.n. inoculation of each indicated virus were monitored daily for 14 days in ferrets. Virus titers in the nasal turbinates (C) and lungs (D) of 3 ferrets per group sacrificed on days 1 (black bars), 3 (open bars), and 5 (grey bars) p.i., respectively are expressed as mean ± SE log10 TCID50/g of tissue. The dashed horizontal line indicates the lower limit of detection.

