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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Apr 24;42(7):1201–1209. doi: 10.1016/j.biocel.2010.04.009

Fig. 6.

Fig. 6

Effect of Rab1 on β-ARs mediated ERK1/2 activation in RPMVECs by Western blotting analysis using phospho-specific ERK1/2 (ERK1/2-P) antibodies. RPMVECs cultured in six-well dishes were infected with control, Rab1WT, or Rab1N124I lentivirus at an m.o.i. of 20 for 2 days. Representative blots of ERK1/2 (top) and total ERK1/2 expression (bottom) are shown. (A) The RPMVECs were stimulated at 37°C with ISO (10 μM). (B) The cells were treated with ISO plus ICI 118,551 (10 μM). (C) The cells were treated with ISO plus atenolol (10 μM). (D) Quantitative data of ERK1/2 phosphorylation normalized to the total ERK1/2 expression. The data shown are the percentage of the mean value obtained from the RPMVECs infected with control lentivirus and presented as the means ± S.E. at three separate experiments. The groups labed ‘-’ were did not treated with ISO, ISO /ICI and ISO / Atenolol (The value of ERK1/2 were not shown). * p < 0.05 versus RPMVECs infected with control lentivirus.