Detection of NOTCH3 and CD146 in human apical papilla. (A) Immunohistochemical staining of the apical papilla, showing NOTCH3 staining is associated with blood vessels. (B) Staining for NOTCH3, (C) control. (D–H) Immunoflourescence staining of the apical papilla showing blood vessels stained for D) NOTCH3 (green), (E) CD146 (red), (F) co-localization of NOTCH3 and CD146, (G) DAPI (blue), (H) co-localization of NOTCH3, CD146 and DAPI. Data are representative of independent experiments of samples from 2 donors each performed in triplicate.