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. 2015 May 30;2(3):261–267. doi: 10.1016/j.gendis.2015.05.003

Fig. 2.

Fig. 2

Expression of NOTCH3 and JAG1 in SCAP at passage 3. (A, B) Immunocytochemical staining of SCAP, showing positive staining for NOTCH3 in the perinuclear region (indicated by the white arrow in A) and in the nucleus (yellow arrow head in A). (C–H) Immunoflourescence staining of SCAP showing positive staining for NOTCH3 (green) and JAG1 (red). Note that NOTCH3 is located in the perinuclear region and in the nucleus while JAG1 only in the cytplasm and perinuclear region. In (C–E), isolated single cells showing both NOTCH3 and JAG1 in the same cell. Similar finding is in (F–H) when cells are in contact with each other. Data are representative of independent experiments of samples from 3 donors each performed in triplicate.