Fig. 3.

Flow cytometry analysis of SCAP at passage 3. (A) Representative images of SCAP of ∼80% confluence before subjected to flow cytometry. (B) SCAP double staining with NOTCH3 and STRO-1: 7% is expressing NOTCH3, 15.8% expressing STRO-1 and 1.5% expressing both STRO-1 and NOTCH3. (C) Flow cytometry dot plots showing triple- staining of SCAP for CD146 (red), STRO-1 (purple) and NOTCH3 (blue). Note the triple positive population of cells are shown in green. Data are representative of 2 experiments performed in triplicate.