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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: RSC Adv. 2016 Jan 12;6(11):8980–8991. doi: 10.1039/C5RA26022A

Figure 3. Cells aggregate inside multiwell plates with embedded PDMS microwell inserts.

Figure 3

(a) 344SQ-GFP cell aggregates in a 12-well plate with 1.2 mm center-to-center spacing at ~80 microwells/cm2. Aggregates can be imaged directly inside microwells from below; scalebar=2,000 μm (left), scalebar=500 μm (middle). Aggregates can also be seen in a cross-sectional side view; scalebar=500 μm (right). (b) Labelled C4-2 cells in a 12-well plate with 0.25 mm center-to-center spacing at ~1,800 microwells/cm2; scalebar=2,000 μm (left), scalebar=200 μm (middle), scalebar=200 μm (right). (c) 344SQ-GFP aggregates in microwells from an Ommnitray (single-well plate with multiwell plate footprint) demonstrates feasibility of microwell production scale-up; scalebar=2,000 μm. (d) Labelled 344SQ MCAs isolated from microwells and the maximum intensity projection of a single MCA imaged with confocal microscopy (inset); scalebar=200 μm, scalebar=25 μm (inset). (e) MCA Maximum Feret Diameter histogram for microwells seeded with cells at 10, 25, and 75 cells/microwell and harvested after one day of aggregation. (f) MCAs from microwells seeded at 75 cells/microwell were filtered with a 40 μm cell strainer.