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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: RSC Adv. 2016 Jan 12;6(11):8980–8991. doi: 10.1039/C5RA26022A

Figure 4. Cells aggregate in PDMS microwells made by replica molding.

Figure 4

(a) Schematic of non-ablative PDMS microwell fabrication by replica molding. A polyurethane microneedle array (see Fig. 1e) can be cast from a pre-existing PDMS microwell template (Fig. 1d). The polyurethane cast can serve as master for successive casting of PDMS microwell arrays without the need for laser ablation. (b) Cross-sectional view of PDMS microwells made by replica molding; scalebar=200 μm. (c) Top view of 344SQ-GFP aggregates (Day 1) in PDMS microwells made by replica molding; scalebar=200 μm. (d) MCA Maximum Feret Diameter histogram for microwells seeded with cells at 10 cells/microwell and harvested after one day of aggregation.