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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: RSC Adv. 2016 Feb 17;6(26):21776–21788. doi: 10.1039/C6RA03706B

Figure 1.

Figure 1

SDS-PAGE of purified recombinant silk-silica chimeric proteins. (a) nh-15mer (~40 kDa), nh-15mer-R5 (~43 kDa), 15mer-ch (~40 kDa) and R5-15mer-ch (~43 kDa), were run on the 4%-12% Bis-Tris acrylamide gel and stained with Simple Blue dye. Marker (M) sizes are indicated on the left. (b) Schematic representation of silk-silica fusion proteins design strategy; His-tag (green box) has been added to spider silk 15mer (blue arrow) at N-terminal end of nh-15mer and nh-15mer-R5 constructs, and C-terminal end of 15mer-ch and R5-15mer-ch constructs; R5 domain (yellow circle) has been added to the C-terminal of nh-15mer-R5 and N-terminal of R5-15mer-ch.