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. Author manuscript; available in PMC: 2016 Mar 15.

Published in final edited form as: RSC Adv. 2016 Feb 17;6(26):21776–21788. doi: 10.1039/C6RA03706B

Calcium crystal deposition by differentiating human mesenchymal stem cell on the recombinant silk and silk-silica films. Calcium crystals (red) staining by Alzarin Red S was performed on hMSCs cells grown on nh-15mer, nh-15mer-R5, 15mer-ch, R5-15mer-ch and TCP four (a) and eight (b) weeks post seeding. Scale bars are 300 µm. (c) Quantification of calcium deposition of hMSCs grown on recombinant silk-silica constructs after 4 weeks (white bars) and 8 weeks (grey bars) in culture. Results for nh-15mer, nh-15mer-R5, 15mer-ch and R5-15mer-ch silk-silica constructs and TCP no osteogenesis and TCP osteogenesis are shown. Data are represented as the average ± standard deviation (n=3, *p < 0.05).

Calcium crystal deposition by differentiating human mesenchymal stem cell on the recombinant silk and silk-silica films. Calcium crystals (red) staining by Alzarin Red S was performed on hMSCs cells grown on nh-15mer, nh-15mer-R5, 15mer-ch, R5-15mer-ch and TCP four (a) and eight (b) weeks post seeding. Scale bars are 300 µm. (c) Quantification of calcium deposition of hMSCs grown on recombinant silk-silica constructs after 4 weeks (white bars) and 8 weeks (grey bars) in culture. Results for nh-15mer, nh-15mer-R5, 15mer-ch and R5-15mer-ch silk-silica constructs and TCP no osteogenesis and TCP osteogenesis are shown. Data are represented as the average ± standard deviation (n=3, *p < 0.05).