Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PPK protein and poly P production of the wild-type, PPK4320 and PPK4320C. Bacteria total protein was extracted from overnight (18 h) LB broth cultures. a PCR verification of intron insertion with the primers ppk-outF and ppk-outR. b Western blot testing of the PPK protein expression. c Poly P levels analysis. Values are expressed as the quantity of poly P (μg) per milligram of total cellular protein. d Examination of the growth curves. *P < 0.05, **P < 0.01
