Table 1.

Primers used for the construction of the ppk mutant and ppk complementation strain

Primer name	Sequence (5′–3′)
ppk-IBS	AAAAAAGCTTATAATTATCCTTAAGAGCCTGAGCCGTGCGCCCAGATAGGGTG
ppk-EBS1d	CAGATTGTACAAATGTGGTGATAACAGATAAGTCTGAGCCGCTAACTTACCTTTCTTTGT
ppk-EBS2	TGAACGCAAGTTTCTAATTTCGATTGCTCTTCGATAGAGGAAAGTGTCT
ppk-outF	CAAATATCCCGGTCTGCATACACAT
ppk-outR	CAATACACCTTCACGTAATGCACCA
EBS universal	CGAAATTAGAAACTTGCGTTCAGTAAAC
Intron3end	CAGAGCCGTATACTCCGAGA
pGMF	ATAAGTACTTTGAGACAATTTACCGAACAAC
pGMR	ATAAGTACTAGAAATGCCTCGACTTC
pkF	ATAGATATCATGTCCCAAGAACGACTCTATATTGATAAAG
pkR	AGAGGATCCTTACGCTCGTGATCCTGGTTGTTC