Fig 4. PE_PGRS33 activates the TLR2-dependent pro-adhesive pathway in macrophages.
pMM0 isolated from C57Bl/6 mice were incubated with wortmannin or with an anti-CR3 antibody (M1/70), or left untreated, and then infected (MOI 1:10) with MtbΔ33 and the complemented strain MtbΔ33::PE_PGRS33HA and intracellular CFUs were determined at 4h post-infection. Results of one representative experiments of at least three assays are shown (*p<0,.001). CFUs were expressed as mean ± SD and were analysed by two-way ANOVA followed by Bonferroni posttest.