Skip to main content
. 2016 Mar 15;11(3):e0151396. doi: 10.1371/journal.pone.0151396

Fig 1. VEGF and sFlt-1 mutant phenotypes. VEGF mutant overexpression of VEGF by RT-PCR and ELISA. sFlt-1 mutant expression of sFlt-1 by RT-PCR.

Fig 1

(A) VEGF littermate mice revealed a normal, pink to yellow phenotype of the intestine. (B) VEGF mutants demonstrated a deeper red color of the intestine. (C) VEGF mutant mice revealed a 7.36 ± 1.49 SEM-fold increase of transgenic VEGF transcript compared to littermates. (D) VEGF mutant mice duodenum displayed a 13.92 ± 2.60 SEM-fold increase of VEGF protein compared to the littermates; N = 3 mice per group; *p<0.05; Error bars = SEM. (E) s-Flt-1 littermate mice with a normal intestine. (F) sFlt-1 mutants demonstrated paler coloration of the intestine. (G) sFlt-1 mutant mice duodenum demonstrated a significant decrease in full length Flt-1 and sFlt-1 transcript at 21 days compared to littermates, suggesting a negative feedback response. N = 3 mice per group; *p<0.001; Error bars = SEM. (H) sFlt-1 mutant small intestine enteroid cultures demonstrated increased expression of full length Flt-1 and sFlt transcript after 3 days of doxycycline administration. N = 25 OU per well; 6 wells; *p = 0.019; Error bars = SEM.