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. 2016 Mar 15;11(3):e0151396. doi: 10.1371/journal.pone.0151396

Fig 4. VEGF mutant villi demonstrated increased angiogenesis and vascular permeability.

Fig 4

(A) Cross-section of littermate duodenum. (B) Cross-section of VEGF mutant duodenum; Scale bars = 100 μm (C) Single RBC counts per individual villus were increased in VEGF mutant mice; N = 3 mice per group; 20–35 villi per mouse were evaluated; *p<0.001; Error bars = SEM. (D) RBCs cluster counts (>3 RBCs in close contact) per individual villus were increased in VEGF mutant mice; N = 3 mice per group; 20–35 villi per mouse were evaluated; *p<0.001; Error bars = SEM. (E) Three-dimensional cross-section of littermate duodenum. (F) Three-dimensional cross section of VEGF mutant duodenum. Nuclei labeled with DAPI (Cyan) and vasculature labeled with FITC-dextran (Yellow); Scale bars = 100 μm. (G) VEGF mutant vasculature displays increased volume compared to littermates; N = 4 mice per group; *p<0.001; Error bars = SEM.