Table 3. Primers and Hydrolysis Probes Used for RT-qPCR.
| Primers | Sequence |
|---|---|
| VEGF-A F | 5’-ACTGGACCCTGGCTTTACTG-3’ |
| VEGF-A R | 5’-TCTGCTCTCCTTCTGTCGTG-3’ |
| sFlt-1 F | 5’-CGACTCACTATAGGGAGACCC-3’ |
| sFlt-1 R | 5’-TGGCCTGCTTGCATGATGTGCTGG-3’ |
| Bmi1 F | 5’-CAAAACCAGACCACTCCTGAA-3’ |
| Bmi1 R | 5’-CCATGATAGGCTTTGATGACTTT-3’ |
| Lgr5 F | 5’-CTTCACTCGGTGCAGTGCT-3’ |
| Lgr5 R | 5’-CAGCCAGCTACCAAATAGGTG-3’ |
| Atoh1 F | 5’-TGCGATCTCCGAGTGAGAG-3’ |
| Atoh1 R | 5’-CTCTTCTGCAAGGTCTGATTTTT-3’ |
| Hes1 F | 5’-TGCCAGCTGATATAATGGAGAA-3’ |
| Hes1 R | 5’-CCATGATAGGCTTTGATGACTTT-3’ |
| Sox9 F | 5’-GAAAGACCACCCCGATTACA-3’ |
| Sox9 R | 5’-TCCGCTTGTCCGTTCTTC-3’ |
| DII1 F | 5’-ACATGTTCCTGCCGACCT-3’ |
| DII1 R | 5’-GGGCTAGGAGCACACTCATC-3’ |
| Wdr43 F | 5’-GGACAGAAGGCAAAATTGGT-3’ |
| Wdr43 R | 5’-TCTCTGGAACCTCATCGTCA-3’ |
| EphB2 F | 5’-TATGCCGCAACGGCTACT-3’ |
| EphB2 R | 5’-GTCTCGTTGACGCTGGAGAT-3’ |
| Bmp4 F | 5’-GAGGAGTTTCCATCACGAAGA-3’ |
| Bmp4 R | 5’-GCTCTGCCGAGGAGATCA-3’ |
| VEcad F | 5’-GTTCAAGTTTGCCCTGAAGAA-3’ |
| VEcad R | 5’-GTGATGTTGGCGGTGTTGT-3’ |
The above are the nucleotide sequences for genes from RT-qPCR and the appropriate hydrolysis probes from the Roche Applied Science Universal Probe Library that were employed in the RT-qPCR reactions.