Fig 4. Fibronectin induces smaller adhesion on low E-cadherin expression OSCC cell line.

L^inv/H^E-cad (A) or H^inv/L^E-cad (B) invasive OSCC were plated on laminin or fibronectin, fixed and stained for E-cadherin and actin, paxillin, vinculin and FAK. White arrows indicate the signal of E-cadherin between cells. Scale bar = 20μm. Data regarding adhesion properties (C) were obtained using Total Internal Reflectance Fluorescent microscopy analysis of H^inv/L^E-cad OSCC cells expressing paxillin-GFP and plated on laminin (light gray) or fibronectin (dark gray). The data shows the assembly speed (μm/sec), adhesion area (μm²), total adhesion area (as % of total protrusion area) and adhesion length (μm). Results are expressed as mean ± SEM. (*) p = 0.05; (**) p < 0.01, according to Student T—test.