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(A) DIV 10 hippocampal neurons were treated with BDNF for 30 minutes, fixed and stained for pERK and pan ERK. (B, C) BDNF application caused an increase in nuclear pan ERK and pERK immunofluorescence levels in WT but not Jacob/Nsmf ko neurons. Two-way ANOVA with Tukey posttest ***p<0.001; **p<0.01, *p<0.05. Scale bar indicates 20μm.
