Figure 5. ADCC activity of ADAM17-deficient patients' NK.
A.-C. NK cells from two healthy donors (CTRL-1 and CTRL-2) and ADAM17 deficient patient (ADAM17) were pre-treated by DMSO or Marimastat and co-incubated with Raji A., HepG2 B. and SK-BR-3 C.. Target cells that were coated or not with the indicated mAbs. IFNγ levels were measured following four hours co-incubation. Values are normalized to the control mAb secretion. *p < 0.03, **p < 0.003, ***p < 0.0005. D.-F.35S labeled Raji cells D., HepG2 cells E., or SK-BR-3 cells F. were incubated with the appropriate mAbs and then incubated with IL-2 activated bulk NK cell cultures derived from the ADAM17 patient and from two healthy control donors as indicated at the X axis, The effector to target ratios was 20:1. Shown is one representative experiment out of two performed. *p < 0.05, **p < 0.02, *** < 0.009 by a two tailed student T-TEST.