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. 2015 Oct 27;6(42):44323–44331. doi: 10.18632/oncotarget.6249

Figure 1. Over-expression of Cx43 on hUCSCs.

Figure 1

Mononuclear cells from human cord blood were obtained by ficoll density gradient and cultured for 2 days alone or with Cx43 vector for Cx43 over-expression (A) Quantification of Cx43 RNA expression normalized to β-Actin. (B) Quantification of Cx43 protein by western blot with antibodies against Cx43 and normalized to β-Actin. Immunofluorescence microscopy of transfected cells labeled with antibodies against Cx34 (red) before transfection (C) and 30 days after transfection (D). Nuclei were labeled with 4,6 diamidino-2-phenylindole (DAPI; blue). (E) Quantification of Cx43 labeling fluorescent intensity normalized to DAPI.