Skip to main content
. 2015 Oct 27;6(42):44323–44331. doi: 10.18632/oncotarget.6249

Figure 2. Cx43-hUCSC improves GJIC among cells.

Figure 2

L615 cells were cultured to 80% confluence on top of a mixture of L615 BM+Cx43-hUCSCs or on top of L615 BM only in a chamber slide. The dye CFDA, is loaded into the chamber (10 μmol/L, 15 min) before FRAP assay. Clusters of 5 to 6 cells (L615 cells) were selected under the microscope with the 20 × objective lens. Cells then were photo bleached to 10% of the original fluorescence intensity. Cell fluorescence intensity recovery was quantified after photo bleaching and plotted against time.