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. 2015 Oct 27;6(42):44323–44331. doi: 10.18632/oncotarget.6249

Figure 4. Increasing levels of activated caspase in L615 cells when co-cultured with BMSCs.

Figure 4

L615 cells were cultured alone, or co-cultured with either Cx43-hUCSC or hUCSC for three hours. Then L615 cells were harvested and the levels of activated caspase 3, 6 and 7 were quantified by Western blotting. (A) Levels of active caspase 7 were significantly increased in the conditions where cells were co-cultured with hUCSC or with Cx43-hUCSC compared to that of L615 cells grow alone. Activated caspase 7 was increased 3 fold in the hUCSC co-culture compared to that of L615 alone. When L615 cells are co-cultured with Cx43-hUCSC, active caspase 7 is increased up to 7 fold above that of L615 alone. Representative blots for Caspase 7 and β-Actin are shown. (B) Levels of active caspase 3 were significantly increased in L615 cells co-cultured with Cx43-hUCSC, but not in co-cultured with hUCSC. *indicates p < 0.05 (N = 3) (C) There is no difference in the levels of active caspase 6 among different culture conditions.