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. 2015 Oct 27;6(42):44360–44372. doi: 10.18632/oncotarget.6246

Figure 3. RNase L depletion by RNAi increases migration of DU145 cells.

Figure 3

(A) RNase L and β-actin levels in DU145 cells transfected with control or RNase L siRNA oligonucleotides as determined in immunoblots. (B) RNase L activity determined by rRNA cleavage in response to pIC transfection monitored in RNA chips (Agilent). (C−E) Migration of DU145 control shRNA- and RNase L shRNA-expressing cells by either (C) transwell assays or by (D, E) scratch wound healing assays. AU, arbitrary units. C–E. Data are shown as the mean ± SD each from a single experiment with 3 technical replicates. **p < 0.001 by Student's two-tailed t tests. The experiments were conducted 3 times (biological replicates) with similar results.