Figure 6. Treatment with pharmacological inhibitor ICI reduces osteoblastic lesion development and lung metastasis.

A. Whole body bioluminescence imaging was performed to measure tumor growth over time in the tibiae. B. and C. Representative images from X-ray radiography and H&E staining are shown. Arrow shows new bone formation inside the tibia. D. Bone area to total tissue area was quantified with BIOQUANT analysis software. Data are presented as mean ± sem with each dot representing one tibia. E. Lung metastasis incidence was confirmed in the excised whole lungs after the termination of experiment at 7 weeks. ****P < 0.0001.