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. 2015 Oct 30;6(42):44437–44451. doi: 10.18632/oncotarget.6295

Figure 6. Effect of p50 siRNA transfection or NF-κB inhibitor (PAO) on BV-treated colon cancer cell growth and expression of DR4 and DR5.

Figure 6

A. Effect of p50 knockdown with p50 siRNA transfection on the inhibitory effect of BV in colon cancer cell growth. Cell viability was determined by MTT assay. *p < 0.05 indicates statistically significant differences from control cells. #p < 0.05 indicates significantly different from BV treated cells. B. Effect of p50 siRNA transfection on the expression of p50 translocation to nucleus. Values under Western band indicate the density of band. Each band is representative for three experiments. C. Effect of NF-κB inhibitor (PAO) on BV-treated colon cancer cell growth. Cells were pretreated with NF-κB inhibitor, PAO (0.1 μM) for 1 h and then were treated with BV for 24 h. Cell viability was determined by MTT assay. *p < 0.05 indicates statistically significant differences from control cells. #p < 0.05 indicates significantly different from BV treated cells. D. Effect of NF-κB inhibitor (PAO) on the expression of DR4 and DR5. Cells were pretreated with PAO (0.1 μM) for 1 h and then were treated with BV for 24 h, and whole cell extracts were analyzed by Western blotting using DR4, DR5 and β-actin (internal control). Values under Western band indicate the density of band. Each band is representative for three experiments.