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. 2015 Nov 9;6(42):44703–44713. doi: 10.18632/oncotarget.6300

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Copyright: © 2015 Demosthenous et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Immunoprecipitation was performed in HEK293^PTPN6/WT, HEK293^PTPN6/N225K and HEK293^PTPN6/A550V cells with PTPN6 antibody, followed by immunoblotting with STAT3 antibody (n = 2). B. JAK1, JAK2 and JAK3 were immunoprecipitated from lysates of the transfected HEK293^PTPN6/WT, HEK293^PTPN6/N225K and HEK293^PTPN6/A550V cells and the immuno-complexes were examined for presence of PTPN6 (n = 3).