Figure 5. The effect of PTPN6^WT, PTPN6^N225K and PTPN6^A550V on STAT3 transactivation activity, STAT3 direct downstream targets and cell proliferation.

A. STAT3 transactivation activity was evaluated by luciferase reporter assay in the HEK293T stably transfected cells. Briefly the HEK293T were transiently transfected with a STAT3 luciferase reporter and luciferase assay was performed in empty vector, PTPN6^WT and mutants PTPN6^N225K and PTPN6^A550V. Bars represent mean ± SD from 3 different experiments (*P < 0.05). B.-C. Effect of PTPN6^WT, PTPN6^N225K and PTPN6^A550V on STAT3 direct targets, Mcl-1 and survivin B.; Bcl-2 and c-Myc C. were evaluated by western blotting (n = 3). D. MTT assay was performed in the HEK293T stably transfected cells as the readout for cell proliferation. Bars represent mean ± SD from 3 different experiments (**P < 0.005).