Figure 3. Stromal cells activate several protein kinases and transcription factors in CLL cells exposed to ATO.
8–10 × 106 CLL cells in RPMI/0.1% FBS were cultured in suspension or over HS-27A cells for 2 h at 37°C. 2 μM ATO was added or not and cells further incubated for 24 h. Protein kinases (A) and transcription factors (B) were analyzed by Western blotting. The results from two representative samples and the quantitation of all samples analyzed, after normalizing control values to 1, are shown. Vinculin was also analyzed as a control for protein loading. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.