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. 2015 Dec 23;6(42):44927–44940. doi: 10.18632/oncotarget.6743

Figure 2. TMSB4Y is lost in male breast cancer and expressed in normal male breast tissue.

Figure 2

A. Regional Y loss was assessed by STS-PCR that was repeated 5 times using 5 serial slides from matched tumor and normal FFPE gDNA from three patients as described in the text. A summary of each STS-PCR is depicted as a decagon, where a positive reaction is red for tumor, and the corresponding normal somatic control is blue. B. Results summary for a patient who retained the Y chromosome in his breast cancer by FISH, but demonstrated consistent loss of the STS-PCR marker S17, a region containing the TMSB4Y gene, in tumor but not in adjacent normal tissue (outlined in green). Primer sets S13 and S27 were uninformative (shaded out) due to non-specific amplicons and lack of amplicons (amplicon size too large as described in text), respectively. C. Immunohistochemistry labeling for TMSB4Y was performed on normal breast tissue (left) and corresponding breast cancer (right) from a male breast cancer patient with known Y chromosome loss by FISH. Original magnification: 20X.