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. 2015 Dec 23;6(42):44927–44940. doi: 10.18632/oncotarget.6743

Figure 5. TMSB4Y expression reduces cell proliferation.

Figure 5

Parental TetHyg2.5, a stably transfected empty vector (EV) control cell line and the two TMSB4Y Dox-inducible clones, TmY1 and TmY2, were placed in un-induced (−Dox) and induced (+Dox) media conditions and used for cell proliferation assays and FACS analysis. A. TmY1 and TmY2 exhibited ~30% of reduced proliferation upon Dox induction (*p < 0.05) relative to un-induced cells after 6 days of growth. B. Relative reduced cell proliferation after Dox-induction in both TmY1 and TmY2 was observed with prolonged culture as measured by cell counts at Days 3, 7, 13, and 18. C. TMSB4Y expression reduced the relative metaphase cell fraction in TMSB4Y expressing cells (TmY1) after Dox induction, but not in empty vector (EV) control cells after 48 hours and GFP-sorting for cell cycle analysis.