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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Life Sci. 2016 Feb 6;148:31–40. doi: 10.1016/j.lfs.2016.02.015

Figure 7.

Figure 7

Survival curves of G. mellonella larvae infected with the A. nosocomialis parent or the ΔkatEΔkatG mutant strain. Overnight bacterial culture were inoculated into fresh medium and allow to grow for 3 h. Cells were collected by centrifugation, washed and resuspended in PBS to a concentration of 2× 107 CFU/ml. Each G. mellonella larva was injected with 1× 105 CFU of the A. nosocomialis parent or the ΔkatEΔkatG mutant strain (in 5 µl PBS), or injected with 5 µl PBS through the last left proleg. Injected larvae were incubated at 37°C in humidified environment. Survival was monitored for 8 days. Survival curves represent data compiled from 4 independent experiments. The survival curves were compared by log-rank test. *, p<0.05.