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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Behav Neurosci. 2016 Feb 25;130(2):137–155. doi: 10.1037/bne0000135

Figure 2. Physiological assays of DREADD effects.

Figure 2

A, Dentate gyrus granule neurons were infected with lentivirus expressing DREADD receptors. Left, GFP-T2A-HM3Dq expressing neuron recorded in slice given subthreshold current pulses every 5 seconds exhibiting robust bursting response to bath CNO application. Right, HM4Di expressing neuron receiving suprathreshold current injections every 5 seconds stops firing in response to bath application of CNO. B, Entorhinal cortical fast spiking interneuron infected with AAV HM3Dq-mCherry under current clamp showing minimal spiking in response to current steps (left) then a robust excitatory response to CNO application (right). In this case, the CNO was produced internally rather than acquired from an external vendor. C, Example of ventral pallidum neuron recorded in a behaving animal using tetrodes following earlier injection of AAV8-hM4Di; this neuron exhibited a short-latency, long-duration suppression of spontaneous firing activity following i.p. CNO application (short pause in recording for injection). Top, raster of spiking activity. Bottom, histogram in 5-min time windows.