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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Behav Neurosci. 2016 Feb 25;130(2):137–155. doi: 10.1037/bne0000135

Figure 3. Example of cell type-specific expression of DREADDs.

Figure 3

A, Tiled 5× coronal images showing bilateral expression of hM3Dq in the midbrain (AAV2-DIO vector in TH-Cre rat). mCherry (red; DS Red immunostain) shows DREADD expression superimposed on tyrosine hydroxylase-positive (TH+) neurons (green; TH immunostain) in the ventral midbrain. Overlap indicated in yellow, showing restricted DREADD expression in ventral tegmental area (VTA) and not in lateral substantia nigra pars compacta (SNc). B, 60× image of neuron co-expressing hM3Dq (red) and TH (green). Note membrane bound, punctate staining of mCherry-fused hM3Dq receptors.