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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cell Immunol. 2015 Dec 19;302:1–10. doi: 10.1016/j.cellimm.2015.12.007

Figure 1.

Figure 1

Induction of nitric oxide pathway by high sodium chloride in breast cancer cells. (A) Cell viability analysis of MDA-MB-231 in the presence of varying NaCl concentration (0.1 -0.3 mM). It is important to note that 0.1 M NaCl is the basal sodium chloride concentration in the RPMI1640 culture media and therefore 0.1 M NaCl refers to basal media treatment control group. (B) Cell viability analysis of MDA-MB-231 in the presence of varying interleukin (IL-)17 concentration (0.1 - 500 nM) in basal (0.1 M NaCl) media. (C) Lack of cell death with NaCl (0.15 M) and IL-17 (0.1 nM) combination. (D) Cell viability was similar among various breast cancer (MDA-MB-231, MCF-7) and normal breast (MCF10A) cell lines with NaCl (0.3 mM) and IL-17 (0.1 nM) combination; (E) iNOS, inducible nitric oxide synethsase (E), nitric oxide (F) and RNS, reactive nitrogen species (G) expression in all three cell lines mentioned above following treatment with NaCl (0.15 M) and/or IL-17 (0.1 nM in basal 0.1 M NaCl media). Data represented mean values ± SEM from five independent experiments. Student-t-test performed for statistical analysis (significance p<0.05).