Figure 2.
High sodium chloride induced reactive oxygen species in breast cancer cells. (A) Western blot analysis of anti-oxidant enzyme (SOCS, SOD1, SOD2) expression following treatment with NaCl (0.15 M) and/or IL-17 (0.1 nM in basal 0.1 M NaCl media) in three cell lines; (B) ROS, reactive oxygen species expression in all three cell lines mentioned above following treatment with NaCl (0.15 M) and/or IL-17 (0.1 nM). (C-E) mRNA analysis for SOCS, SOD1, SOD2 expression in various breast cancer cell lines (MDA-MB-231, MCF-7 and MCF-10a) following co-treatment with high salt (0.15 M NaCl) and 0.1 nM IL-17. Data represented mean values ± SEM from five independent experiments. Student-t-test performed for statistical analysis (significance p<0.05).