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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cell Immunol. 2015 Dec 19;302:1–10. doi: 10.1016/j.cellimm.2015.12.007

Figure 5.

Figure 5

ERK-1/2 protein kinase upregulation in γENaC induced inflammatory stress. (A) Western blot (phospho-blot) analysis of signaling factors (ERK-1/2, p-ERK-1/2, p38, c-Jun) following treatment with NaCl (0.15 M M) and/or IL-17 (0.1 nM in basal 0.1 M NaCl media) in three cell lines; (B) Western blot (phospho-blot) analysis of protein kinase, (p-)ERK-1/2, following siRNA knock-down of γENaC and/or lipid rafts in three cell lines; (C) Efficient knock-down of ERK-1/2 by specific siRNA, while scramble siRNA does not decrease expression of ERK-1/2. (D-F) Inhibition of nitric oxide (C), RNS (D) and ROS (E) release following siRNA knock-down of ERK-1/2. Data represented mean values ± SEM from five independent experiments. Student-t-test performed for statistical analysis (significance p<0.05).

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