Fig. 1.

Sialylation of relevant integrins on MDA-MB-231 and MCF-7 cells. For lectin affinity assay, 300 μg cell lysate of MDA-MB-231 and MCF-7 cells were harvested and incubated with 50 μg/ml biotinylated SNA at 4°C overnight. Streptavidin agarose beads were then added for additional 4 hours incubation at 4°C. Beads were washed and boiled before loading to the gel. Loading control for lectin affinity assay was collected before adding biotinylated SNA. For western blotting, 100 μg cell lysate was loaded. This figure is representative of 3 independent experiments.