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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cell Immunol. 2015 Dec 30;302:11–18. doi: 10.1016/j.cellimm.2015.12.009

Fig. 1. Increase frequency of IFNγ producing T cells in the lungs of auxotroph immunized TLR2/9DKO mice.

Fig. 1

WT, TLR9KO and TLR2/9DKO mice were vaccinated subcutaneously with Mtb-auxotroph. 9 weeks post-vaccination, single cell suspension was prepared from lungs, spleen and draining lymph nodes. Frequency of IFNγ+ cells was measured as number of spots upon ex vivo stimulation with Mtb-infected dendritic cells from lungs (a), spleen (b) and lymph nodes (c). Percent ESAT6+CD4+CD44hiCD62Llo T cells from the lungs were quantitated (d, e). Each group includes 4–5 mice per time point. (Data represented as mean+/− SEM, *** p<0.001 and ** p<0.01)