FIGURE 4.
Different scanning configurations in LOT/MFMT. (a) Raster scanning mode accepts an excitation light (blue color) through a beam splitter (BS). The light is scanned in two dimensions with two scanning mirrors (SM). The emission light (green) follows the similar path of excitation, is de-scanned by the SMs, and then captured by a detector (D). (b) Line scanning mode utilizes line sheet illumination thus one scanning mirror (SM) is sufficient to cover the entire field of view. (c) Point scanning hosts stationary optical elements. In order to cover a field of view, while sample itself is translated with a micro stage.