Figure 2. Scheme of the NCI-60 pharmacogenomic analysis.
(i) Drug sensitivity data were filtered using the ‘cell-line selectivity’ metric, followed by model-based clustering and consolidation of clustering into 18 clusters. (ii) 13,748 genes with larger dispersion in expression were selected based on IQR of their expression across the NCI-60 cell line panel. (iii) The Spearman correlation coefficient between drug sensitivity of each lethal compound cluster and the transcriptional profile of each gene across the NCI-60 panel was computed. (iv) The correlation coefficients between compound clusters and genes were used to compute GO enrichment analysis for each compound cluster.