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All of the HPV- LCNECs were positive for the neuroendocrine marker synaptophysin (A) and negative for the squamous marker p63 (B). All of the HPV-related (and 3 of the HPV-negative) LCNECs were positive for p16 by immunohistochemistry (C). The HPV-related LCNECs were defined by the presence of nuclei signals by DNA in situ hybridization for high-risk HPV (D).
