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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Methods. 2015 Oct 23;97:3–10. doi: 10.1016/j.ymeth.2015.10.008

Figure 1. Schematic of DNA Illumina amplicon.

Figure 1

The amplicon includes an adapter sequence, priming regions, a barcode and the aptamer sequence with two constant regions flanking a variable region. Single end read sequence (Read 1) will begin with the barcode and may include only a partial sequence of the 3′ constant region if the read length does not extend through the 3′ constant region. Paired end read (Read1 and Read 2) will include sequence information from both ends of the amplicon. Pre-processing HTS aptamer data involves isolating the variable region sequence and eliminating reads with errors.