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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Cerebellum. 2016 Aug;15(4):509–517. doi: 10.1007/s12311-015-0720-6

Figure 4. Intracellular localization of TERT is altered by both DNA damage and excitotoxic glutamate.

Figure 4

A–C. Representative image of a cerebellar section immunostained for TERT (red) and COX4 (green), and counter stained with the nuclear DNA stain DAPI (blue). B. DAPI channel alone. The lines represent the region of interest (ROI) that was used to distinguish the nucleus. C. Binary image of mitochondria that was generated by employing the same threshold on all COX4 images from all treatments. This image was used in order to determine the ROI that represent mitochondrial localization. D. The local expression of TERT was measured by the average red fluorescence intensity (TERT staining) in each selected region (as shown in B and C). The intensity in the nucleus was normalized to the blue fluorescence intensity and the intensity in the mitochondria was normalized to the green fluorescence intensity. E. The fraction (% of total staining) of TERT present in the nucleus and mitochondria was determined by dividing the sum of the red fluorescence intensity (TERT staining) in the ROI represent nucleus or mitochondria (as shown in B and C) to the red fluorescence intensity in the full image. Values are mean±SEM (n=7 untreated, n=9 X-ray radiation and n=4 Glutamate-treated). *p<0.05, t-test. Scale bar=20μm