Skip to main content
. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Life Sci. 2016 Feb 11;148:106–111. doi: 10.1016/j.lfs.2016.02.047

Figure 4.

Figure 4

Effect of daily manganese (Mn) supplementation on hypothalamic cyclooxygenase 2 (COX2) protein levels in prepubertal female rats after short term ALC exposure.

The same four treatment groups of animals as described in Figure 1 were used. Panel A) Representative immunoblot of the 72 kDa COX2 protein isolated from the medial basal hypothalamus of prepubertal female rats are shown from the CON+SAL group (lanes 1-3); the CON+Mn group (lanes 4-6); the ALC+SAL group (lanes 7-9); and the ALC+Mn group (lanes 10-12). Panel B) Composite graph represents the densitometric quantitation of all the bands from 2 blots that correspond to the COX2 protein. Note that animals in the ALC+Mn group showed restored COX2 protein synthesis. a vs b, p<0.01; a vs c, p<0.05; b vs. c, p<0.01; b vs d, p<0.05; c vs d, p<0.05; Bars with same letters are not different. N=6 animals/group.